Chromatin Immunoprecipitation (ChIP) of Histone Modifications from <em>Saccharomyces cerevisiae</em>
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چکیده
منابع مشابه
Chromatin immunoprecipitation (ChIP) method for non-model fruit flies (Diptera: Tephritidae) and evidence of histone modifications
Interactions between DNA and proteins located in the cell nucleus play an important role in controlling physiological processes by specifying, augmenting and regulating context-specific transcription events. Chromatin immunoprecipitation (ChIP) is a widely used methodology to study DNA-protein interactions and has been successfully used in various cell types for over three decades. More recentl...
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Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription factor interacts with a candidate target gene and is used with equal frequency to monitor the presence of histones with post-translational modifications at specific genomic l...
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Chromatin modifiers and histone modifications are components of a chromatin-signaling network involved in transcription and its regulation. The interactions between chromatin modifiers and histone modifications are often unknown, are based on the analysis of few genes or are studied in vitro. Here, we apply computational methods to recover interactions between chromatin modifiers and histone mo...
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Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at different developmental stages. Epigenetic regulation contributes to a broad spectrum of biological processes, including cellular differentiation during embryonic development and homeostasis in adulthood. A critical strategy in epigenetic stud...
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Histone epigenetic modifications are one of the frequently observed epigenetic alterations in leukemic cells. To efficiently identify histone acetylation status of a number of gene promoters in chronic lymphocytic leukemia (CLL) specimens, we performed a combination of chromatin immunoprecipitation (ChIP) and PCR array. ChIP by an antipan acetylated H4 histone antibody was followed by a PCR amp...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/57080